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Getting started

  • What xPlant is for
  • First plant and explant workflow

Tissue culture concepts

  • Plants, explants, and stages
  • Media recipes and additives
  • Contamination tracking basics
  • Intro to tissue culture
  • Tissue culture basics

App workflows

  • Record a transfer
  • Batch Tracker
  • Create and use SOPs
  • Comments and team context
  • Lab rooms

Protocols

  • Protocols and SOPs — what's the difference?
  • Sterilization and aseptic technique
  • Media preparation protocol

Lab tools

  • QR labels and the in-app scanner

Developer tools

  • Developer SDK & API

Account and support

  • Privacy and data controls
  • AI features and smart suggestions

Tissue culture concepts

Plants, explants, and stages

xPlant uses three separate record types — Plants, Explants, and Stages — to model the full lifecycle of tissue culture work. Understanding how they relate makes the dashboard much easier to read.

Plants

A Plant is the named parent organism — the source of all culture material in xPlant. It holds a species, cultivar, photo, origin notes, and any tags or labels you apply. It also carries its own stage history (Mother Block, Production, Field, etc.) that reflects the condition of the parent plant itself, not its in vitro derivatives.

Plants are permanent records. Even when all its culture material has been discarded, the plant record stays in your library so you have a complete history of what was worked on and when.

Plant stage options

Mother BlockSource plant held in the lab for repeated explant production.
PropagationActive vegetative multiplication — cuttings, divisions, or nodes.
ProductionScale-up phase producing batches for transfer or sale.
AcclimationTransitioning rooted material from controlled to ambient conditions.
GreenhouseEstablished in greenhouse conditions, growing under natural light.
FieldPlanted out in the ground or large containers.
Cold StorageHeld dormant for later use.
QuarantineIsolated for health monitoring or entry screening.
DiscardedCulture material that has been removed or lost.

Explants

An Explant is a piece of tissue taken from a parent plant for in vitro propagation. Each explant has its own label, its own stage history, and its own contamination and observation record. It is always linked to one parent plant.

When you initiate three vessels from the same mother plant in one session, create three separate explants — one per vessel or tissue type. This lets you track each culture independently: Vessel A might contaminate while Vessels B and C continue successfully.

Explant stage options

PendingExplant created but not yet initiated.
Explant InitiationTissue placed on media for the first time.
Surface SterilizationSterilization step before or after initiation.
MultiplicationActive shoot or callus multiplication.
Shoot ElongationElongating shoots before rooting.
Root InductionApplying rooting stimulus.
RootingActive root formation on shoots.
AcclimatizationWeaning from sterile to ambient humidity.
Hardening OffFinal transition before outdoor or greenhouse placement.
Ready for TransferMaterial staged for the next destination.
QuarantineHeld for observation or screening.
DiscardedCulture lost, contaminated, or discarded.

Stages

A Stage entry records a single point in the lifecycle of a plant or explant. Each entry captures:

  • Stage type (e.g. Multiplication, Rooting)
  • Status (Active, Completed, Failed, etc.)
  • Entry date and optional start / completion timestamps
  • Observation notes from the bench
  • Room or physical location
  • Optional media recipe reference
  • Optional SOP reference

Stage entries stack chronologically into a timeline. The current stage is always the most recent. Previous stages are never overwritten — they form a permanent audit trail.

The key distinction

Plant stages track the condition of the mother plant — is it in production, in quarantine, or discarded? Explant stages track the state of the tissue culture process — is this culture at initiation, rooting, or acclimatization?

A plant can be active as a Mother Block while its explants are simultaneously at Multiplication, Rooting, and Acclimatization. Each record answers a different question.

Related guides

First plant and explant workflowRecord a transferContamination tracking basicsMedia recipes and additives