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Community media library

Community Media Recipes

Explore plant tissue culture media recipes shared publicly by growers and laboratories. Compare formulations, ingredients, and notes before evaluating ideas for your own documented workflows.

Shared by the community

Browse Community Recipes

Every recipe was shared publicly by its owner. Open a recipe to review its formulation, ingredients, preparation notes, and community discussion.

A community reference Shared recipes are grower-contributed records, not verified instructions or guaranteed outcomes. Review the details against your own SOPs, local rules, and safety requirements before use.

15 public recipes
Community recipe

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Murashige & Skoog (MS) Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa M0222 — original MS concentration (1962)

18 ingredients
Cobalt chloride hexahydrate (CoCl₂·6H₂O)Cupric sulfate pentahydrate (CuSO₄·5H₂O)Ferric sodium EDTA (FeNaEDTA)+15
Open recipe
Community recipe

Gamborg B5 Basal Salts

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa G0209 — Gamborg B5 medium

13 ingredients
Cobalt chloride hexahydrate (CoCl₂·6H₂O)Cupric sulfate pentahydrate (CuSO₄·5H₂O)Ferric sodium EDTA (FeNaEDTA)+10
Open recipe
Community recipe

McCown Woody Plant Medium (WPM) with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa M0220 — McCown Woody Plant medium

17 ingredients
Cupric sulfate pentahydrate (CuSO₄·5H₂O)Ferric sodium EDTA (FeNaEDTA)Boric acid (H₃BO₃)+14
Open recipe
Community recipe

Hosta Rooting Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7 and omits cytokinin.

Source: Plantocol — Hosta protocol

4 ingredients
Murashige & Skoog basal mediumSucroseIndole-3-butyric acid (IBA)+1
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Community recipe

Schenk & Hildebrandt (SH) Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Formulated for callus induction and cell suspension culture in both monocots and dicots. High potassium and phosphate relative to MS.

Source: Duchefa S0225 — Schenk & Hildebrandt basal salt medium

16 ingredients
Calcium chloride (CaCl₂)Potassium nitrate (KNO₃)Magnesium sulfate (MgSO₄)+13
Open recipe
Community recipe

DKW/Juglans Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Developed for Juglans (walnut) rootstock and widely adopted for other woody species that respond poorly to MS.

Source: Duchefa D0247 — DKW/Juglans medium including vitamins

16 ingredients
Calcium chloride (CaCl₂)Calcium nitrate dihydrate (Ca(NO₃)₂·2H₂O)Potassium dihydrogen phosphate (KH₂PO₄)+13
Open recipe
Community recipe

Nitsch & Nitsch Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. The medium used to raise haploid Nicotiana plants from pollen grains; the standard starting point for anther and microspore culture.

Source: Duchefa N0224 — Nitsch medium including vitamins

18 ingredients
Calcium chloride (CaCl₂)Potassium dihydrogen phosphate (KH₂PO₄)Potassium nitrate (KNO₃)+15
Open recipe
Community recipe

Anderson's Rhododendron Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Reduced-nitrogen formulation for ericaceous and other acid-loving woody plants — rhododendron, azalea, blueberry, raspberry.

Source: Duchefa A0202 — Anderson's Rhododendron including vitamins

16 ingredients
Calcium chloride (CaCl₂)Potassium nitrate (KNO₃)Magnesium sulfate (MgSO₄)+13
Open recipe
Community recipe

Knudson C Orchid Basal Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa K0215 — Knudson C Orchid medium

8 ingredients
Ferrous sulfate heptahydrate (FeSO₄·7H₂O)Manganese sulfate monohydrate (MnSO₄·H₂O)Calcium nitrate (Ca(NO₃)₂)+5
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Community recipe

African Violet Initiation Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.6–5.8.

Source: Plantocol — African Violet protocol

5 ingredients
Murashige & Skoog basal mediumSucrose6-Benzylaminopurine (BAP)+2
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Community recipe

African Violet Rooting Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation is hormone-free and targets pH 5.6–5.8.

Source: Plantocol — African Violet protocol

3 ingredients
Murashige & Skoog basal mediumSucroseAgar
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Community recipe

Monstera Establishment Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7.

Source: Plantocol — Monstera protocol

3 ingredients
Murashige & Skoog basal mediumSucroseAgar
Open recipe
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